Human Leucocyte Antigen B27, Histocompatibility Antigen (HLA B-27)
- The patient serum is needed.
- The random sample can be taken, no special preparation is needed.
Purpose of the test (Indications)
- To diagnose Ankylosing spondylitis.
- HLA B27 is also advised when the patient has:
- Joint pain and swelling.
- The stiffness of the joints.
- HLA Antigen typing may be done in Bone marrow or kidney transplantation.
- The major histocompatibility antigen in humans belongs to the presence of these antigens on the surface of white cells, and so-called HLA (human leukocyte antigen).
- These are present in the nucleated cells but mostly detected on lymphocytes.
- These antigens are labeled at HLA- A, B, C, and D.
- The presence or absence of these genes is present on chromosome 6.
- HLA-B27 is found in 90 % of the patient with ankylosing spondylitis.
- HLA-B27 is present in the normal population as well in 5 to 7 %.
- HLA-B27 also found in other diseases like Reiter’s syndrome, Anterior uveitis, and Grave’s disease.
|HLA antigen B27 positive||Risk of developing the disease|
|Ankylosing spondylitis||90 times (80 to 90%)|
|Anterior uveitis||15 times (19 to 88%)|
|Reiter’s syndrome||40 times (80%)|
|Septic arthritis||20 times|
|Normal people||5 to 7%|
- HLA-B27 is negative.
Positive HLA-B27 seen in:
- Ankylosing spondylitis.
- Reiter syndrome.
- Grave’s disease.
- Anterior uveitis.
- Chronic active hepatitis.
- Multiple sclerosis.
- Myasthenia gravis.
- Rheumatoid arthritis.
- Celiac disease.
- Juvenile diabetes.