Hepatitis B Virus – Part 4 – Anti-HBS Antibody (ELISA), HBV
- The test is done in the serum.
- How to get good serum: Take 3 to 5 ml of blood in the disposable syringe or in vacutainer. Keep the syringe for 15 to 30 minutes at 37 °C and then centrifuge for 2 to 4 minutes to get the clear serum.
- No special preparation is needed.
- This test can be done on the random sample.
- An Anti-HBS antibody test is done to see the immune status of the patient whether recovered from the infection or not.
- This test may be done in the screening of the population for Hepatitis B prevalence.
- hepatitis B virus is called serum hepatitis.
- HBV incubation period is 5 weeks to 6 months, there is long incubation.
- Patients with Hepatitis B virus infection show hepatitis B surface antigen in the circulation.
- In the case of recovery Antibody against these antigens appears in the blood.
- The patient will be Surface ( HBsAg ) antigen negative.
- The following diagram shows the structure of the HB virus. It has three antigens and respectively there are three antibodies.
The significance of anti-HBs antibody:
- Anti-HBs antibodies are the marker of recovery from the Hepatitis B virus. When positive it means that the patient has recovered from the disease.
- The presence of Anti-Hbs Antibody indicates immunity to Hepatitis B viral infection.
Note Please see more details in the Viral hepatitis B profile.