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FNAC, Fine needle aspiration Cytology (FNAB, Fine needle aspiration biopsy)

FNAC,  Fine needle aspiration Cytology (FNAB, Fine needle aspiration biopsy)
September 18, 2020CytologyLab Tests

Sample sites:

  1. FNA sample may be obtained from:
    1. All parts of the body.
      1. Lymph nodes.
      2. Breast.
      3. Liver.
      4. Respiratory tract.
      5. Oral cavity.
      6. Thyroid.
      7. Genital Tract.
      8. The most common sites are breast, thyroid, and palpable lymph nodes.
  2. FNAC is an inexpensive and less traumatic procedure.
  3. FNA has good results with the help of ultrasound in the Liver and other, abdominal masses.
  4. FNA may be done with the help of a CT scan, mammogram, or ultrasound-guided.

Indications

  1. FNA material gives a cytological diagnosis of the lesion whether benign or malignant.
  2. FNA has excellent results in palpable lumps like breast, or superficial masses.
  3.  FNA material may be subjected to bacterial examination.
  4. FNA is recommended in areas suspected of cystic fluid, nodules, or lumps and in enlarged lymph nodes.

Procedure

  1. This procedure is performed with local analgesia or without it and samples are taken by 25 g needles from the suspected area of the tumor (Our experience is with 25 G needle and we get adequate material).
  2. We made the smear and air dry it before staining.
  3. In the routine, we use Field stain.
  4. Also, we do not give negative pressure unless we see poor yield. Most of the time enough material comes without any aspiration.
  5. After the procedure separates the needle.
  6. Create negative pressure in the syringe and connect the needle.
  7. Push the material on the slide.
  8. Spread the material with another slide.
Diagram showing how to fix the lump

Diagram showing how to fix the lump

How to take sample from the syringe

How to take a sample from the syringe

Important facts

  1. This is one of the best tools for the diagnosis of tumors in expert hands.
  2. FNA is a procedure to obtain diagnostic material for cytologic (Cells) study.
  3. FNA causes minimal trauma to the patient.

Advantages of FNA

  1. It is economical.
  2. No anesthesia required.
  3. No need for hospitalization.
  4. It is less painful.
  5. It is less invasive.
  6. It takes less time as compared to tissue biopsy.
  7. Results are satisfactory in the expert hands.

Reporting

  1. The results can be reported as:
    1. Atypical.
    2. Suspicious of malignancy.
    3. Positive for malignancy.
  2. There are the following possibilities.:
      1. Infectious condition. There will be the presence of characteristic cellular components like poly, lymphocytes, and histiocytes.
      2. Benign conditions. There are characteristic benign cellular changes like uniform nuclei and normal N/C ratio.
      3. Malignant conditions. The cellular changes are typical of malignancy like bizarre cellular appearance and changed N/C ratio, etc.

Reporting of Thyroid FNA

  1. There is a Bethesda reporting system that classified the result into 6 categories.

    1. Nondiagnostic or unsatisfactory.
    2. Benign lesion.
    3. Atypical or undetermined significance or follicular lesion of undetermined significance.
    4. Follicular lesion or suspicious for follicular neoplasm.
    5. Suspicious of malignancy.
    6. Malignant.

Reporting of Breast FNA

  1.  If there is a fluid that will indicate cystic lesion.
  2. FNA will differentiate between the benign or malignant smear.
  3. The FNA is  reported as follows:
    1. Insufficient yield.
    2. There is a benign lesion.
    3. Atypical, probably benign lesion.
    4. Suspicious, probably in situ or invasive carcinoma.
    5. Malignant lesion.

Possible References Used
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