Your source for clinical lab test information.

Field stain consists of:

  1. Field stain A
  2. Field stain B

Thin and thick blood films are made and fixed in methanol for one minute.

Field Stain A

Using Commercially Available Powder

  1. Field stain A commercially available powder = 5 grams
  2. Distilled water heated to 80 °C or keep at 60 °C for 30 min. = 600 mL.
Method
  1. Field stain A (ready made powder).
    1. Mix the powder into the water until it is dissolved.
    2. Filter the solution.
    3. Label the date.

Field stain A from the reagents:

  1. Methylene blue (analytical grade) = 1.6 grams.
  2. Azur = 1 gram.
  3. Disodium dihydrogen phosphate anhydrous = 10 grams.
  4. Potassium dihydrogen phosphate anhydrous = 12.5 grams.
  5. Distilled water = 1000 mL.

Using Reagents for Field stain A

  1. Methylene blue (analytical grade) = 1.6 grams.
  2. Azur = 1 gram.
  3. Disodium dihydrogen phosphate anhydrous = 10 grams.
  4. Potassium dihydrogen phosphate anhydrous = 12.5 grams.
  5. Distilled water = 1000 mL.
  6. Mix both salts in the water.
  7. Add 500 ml from above into bottle containing glass beads.
  8. Add the stain powders (Methylene blue and Azur) both and mix well.
  9. Then add the remainder of the solution.
  10. Mix well and filter into another clean bottle.
  11. Label the bottle Field stain A and also write the date.

Field Stain B

Using Commercially Available Powder

Field stain B commercially available powder = 4.8 grams.
  1. Distilled water heated to 80 °C or keep at 60 °C for 30 min. = 600 mL.
  2. Mix well until it is dissolved.
  3. Filter and label the date.

Field stain B from reagents

  1. Eosin(yellow water soluble) powder = 2.0 grams.
  2. Disodium dihydrogen phosphate anhydrous = 10 grams.
  3. Potassium dihydrogen phosphate anhydrous = 12.5 grams.
  4. Distilled water = 1000 mL.

Preparation of Field stain B from reagents:

  1. Mix both salts in the water.
  2. Add 500 ml from above into bottle containing glass beads.
  3. Add the stain powders Eosin and mix well.
  4. Then add the remainder of the solution.
  5. Mix well and filter into another clean bottle.
  6. Label the bottle Field stain B, and also write the date.

Staining Procedure:

  1. Fill up two Coplin jars or wide-mouth bottles:
    1. Field Stain A (Blue stain).
    2. Field Stain B (Red stain).
  2. Make blood smear on a clean glass slide and it is dried in the air.
  3. Fix in methanol for one minute or get Spray 'Easyfix'.
  4. Dry in the air.
  5. Dip fixed smear to Field Stain B (Red Stain) for 5  to  6 seconds.
  6. Wash in running tap water.
  7. Dip smear into Field Stain A (Blue Stain) for 10 to 30 seconds (adjust it).
  8. Wash in running tap water.
  9. Dry at air and see under oil immersion objective.
    1. Staining time may be adjusted.


Possible References Used

Back to tests