- This test is done on the serum of the patient.
- The random sample can be taken.
- Sample stable at 4 °C for 3 days
- Protect from the light because bilirubin is photo-oxidized (photosensitive).
- Avoid hemolysis and lipemic serum which will give a false result.
- Avoid drugs which will increase the total bilirubin level like:
- Anabolic steroids.
- Antimalarial drugs.
- An antihypertensive drug like methyldopa.
- Oral contraceptives.
- Antituberculous drugs like rifampin.
- Total bilirubin = 0.3 to1.0 mg/dL or 5.1 to 17.0 mmol/L
- Direct bilirubin = 0.1 to 0.3 mg/dL or 1.0 to 5.1 mmol/L
- Indirect bilirubin (total bilirubin minus direct bilirubin level) = 0.2–0.7 mg/dL
- Bilirubin estimation is one of the liver function test.
- It is raised in the hepatic and post-hepatic type of jaundice.
- Clinically jaundice appears when the level of bilirubin is more than 2 mg/dl.
- Direct (or conjugated) bilirubin. The Direct bilirubin dissolves in water (water soluble) and is synthesized in the liver from indirect bilirubin.
Raised level of direct bilirubin is seen in:
- Gallbladder tumors.
- Inflammatory scarring or obstruction of extrahepatic ducts.
- Extensive liver metastasis.
- Dubin-Johnson syndrome.
- Rotor syndrome.
- Drugs may cause cholestasis.
Please see more details on bilirubin Total
Possible References Used
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